Publications

2023

• Didier CM, Fox D, Pollard KJ, Baksh A, Iyer NR, Bosak A, Li Sip YY,  Orrico JF, Kundu A, Ashton RS, Zhai L, Moore MJ, and Rajaraman S. “Fully Integrated 3D Microelectrode Arrays with Polydopamine-Mediated Silicon Dioxide Insulation for Electrophysiological Interrogation of a Novel 3D Human, Neural Microphysiological Construct” ACS Applied Materials & Interfaces, 2023 (https://doi.org/10.1021/acsami.3c05788)

2022

• Iyer NR*, Shin J*, Cuskey S, Tian Y, Nichol N, Doersch T, Seipel F, McCalla SG, Roy S, Ashton RA. “Modular derivation of diverse, regionally discrete human posterior CNS neurons enables discovery of transcriptomic patterns.” [*Co-authorship]  Science Advances, 2022 (doi: 10.1126/sciadv.abn7430)

• Iyer NR* and Ashton RA. "Bioengineering the Human Spinal Cord" Frontiers in Cell and Developmental Biology, 2022 (doi: 10.3389/fcell.2022.942742) [*Corresponding author] 

2015-2021

• Iyer NR*, Shin J*, Cuskey S, Tian Y, Nichol N, Doersch T, McCalla SG, Roy S, Ashton RA. “Modular Derivation And Unbiased Single-cell Analysis Of Regional Human Hindbrain And Spinal Neurons Enables Discovery Of Nuanced Transcriptomic Patterns Along Developmental Axes .” [*Co-authorship]  BioRxiv, 2021 (doi: 10.1101/2021.10.14.464440)

• McCreedy DA, Jalufka FL, Platt ME, Min SW, Kirchhoff NA, Mihaly RME, Butts JC, Iyer NR, Sakiyama-Elbert SE, Crone SA, McDevitt TC. “Passive clearing and 3D lightsheet imaging of the intact and injured spinal cord in mice.” Frontiers in Cellular Neuroscience. 2021 (doi: 10.3389/fncel.2021.684792)

• Fedorchak N, Iyer N, Ashton RA. “Bioengineering tissue morphogenesis and function in human neural organoids.” Seminars in Cell and Developmental Biology, 111, 52-59, 2020. (doi: 10.1016/j.semcdb.2020.05.025)

• Butts JC, Iyer N, White NW, Sakiyama-Elbert SE, McDevitt, TC. “V2a Interneuron Differentiation Methods from Mouse and Human Pluripotent Stem Cells”. Nature Methods, 2019, 15 (1), 181-181. (doi: 10.1038/s41596-019-0203-1)

• Chasman D, Iyer N, Siahpirani AF, Estevez-Silva ME, Lippmann E, McIntosh B, Thomson J, Ashton RS, Roy S. Inference of dynamic, context-specific regulatory networks in early neural differentiation. Cell Systems, 9(2):167-186.e12, 2019, (doi: 10.1016/j.cels.2019.05.012)

• Gamble JR, Zhang E, Iyer N, Sakiyama-Elbert S, Barbour DL. In Vitro Assay for the Detection of Network Connectivity in Embryonic Stem Cell-Derived Cultures. BioRxiv (doi:10.1101/377689)

• Bakooshli MA, Lippmann ES, Mulcahy B, Iyer N, Nguyen CT, Tung K, Stewart BA, Mouly V, Pegoraro E, Ahn H, Ginsberg H, Zhen M, Ashton RS, Gilbert PM. “A 3D model of human skeletal muscle innervated with stem cell-derived motor neurons enables epsilon-subunit targeted myasthenic syndrome studies.” eLife, 2019. (doi: 10.7554/eLife.44530)

• Knight G, Lundin B, Iyer N, Ashton LMT, Sethares WA, Willett RL, Ashton RS. Engineering induction of singular neural rosette emergence within hPSC-derived tissues. eLife, 2018 (doi: 10.7554/eLife.37549)

• Zholudeva LZ, Iyer N, Qiang L, Spruance V, Randelman M, White NW, Bezdudnaya T, Fischer I, Sakiyama-Elbert SE, Lane, MA. Survival and Integration of Enriched Mouse Embryonic Stem Cell-Derived V2a Interneurons Co-Transplanted with Progenitor Populations after Cervical Spinal Cord Injury. Journal of Neurotrauma, 35(24):2883-2903, 2018. (doi: 10.1089/neu.2017.5439)

• Estevez M, Sreeram A, Cuskey S, Fedorchak N, Iyer N, Ashton RS. Single-injection, in ovo transplantation method for broad spinal cord engraftment of human pluripotent stem cell-derived motor neurons. J Neuroscience Methods, 298: 16-23, 2018 (doi: 10.1016/j.jneumeth.2018.01.006)

• Thompson RE, Lake A, Kenny P, Saunders M, Sakers K, Iyer N, Dougherty J, Sakiyama-Elbert SE. Different Mixed Astrocyte Populations Derived from Embryonic Stem Cells have Variable Neuronal Growth Support Capacities. Stem Cells and Development, 26 (22): 1597-1611, 2017(doi: 10.1089/scd.2017.0121)

• Iyer N, Wilems TS, Sakiyama-Elbert SE. Stem Cells for Spinal Cord Injury: Strategies to Inform Differentiation and Transplantation. Biotechnology and Bioengineering, 114(2):245-259, 2017. (doi: 10.1002/bit.26074)

• Iyer, N. “Developing High Purity Embryonic Stem Cell-Derived V2a Interneurons for In Vitro Investigation and Transplantation Following Spinal Cord Injury.” Thesis, Washington University in St. Louis, 2016. (Proquest)

• Iyer N, Huettner JE, Butts JC, Brown CJ, Sakiyama-Elbert SE. “Generation of Highly Enriched V2a Interneurons from Mouse Embryonic Stem Cells.”  Experimental Neurology, 227: 305-316, 2016. (doi: 10.1016/j.expneurol.2016.01.011)

• Marquardt LM, Ee X, Iyer N, Hunter D, Mackinnon SE, Wood M, and Sakiyama-Elbert SE. Finely Tuned Temporal and Spatial Delivery of GDNF Promotes Enhanced Nerve Regeneration in a Long Nerve Defect Model. Tissue Engineering Part A, 21:2852-2864, 2015. (doi: 10.1089/ten.TEA.2015.0311)

• Wilems TS, Pardieck JL, Iyer NR, Sakiyama-Elbert SE. Combination Therapy of Stem Cell Derived Neural Progenitors and Drug Delivery of Anti-Inhibitory Molecules for Spinal Cord Injury. Acta Biomaterialia, 28:23-32, 2015. (doi: 10.1016/j.actbio.2015.09.018)

• Xu H, Iyer N, Huettner JE, Sakiyama-Elbert SE. A puromycin selectable cell line for the enrichment of mouse embryonic stem cell derived V3 interneurons. Stem Cell Research and Therapy, 6:220, 2015. (doi: 10.1186/s13287-015-0213-z)

• Hoben G, Yan Y, Iyer N, Newton P, Hunter DA, Moore AM, Sakiyama-Elbert SE., Wood, MD and Mackinnon SE. Comparison of acellular nerve allograft modification with Schwann cells or VEGF. Hand, 10(3):396-402, 2015 (doi: 10.1007/s11552-014-9720-0)